Getting to Assisi by Train:
From the North, the main line between Florence and Rome has a station
at Terontola (on the branch line to Lake Trasimene, Perugia, Assisi,
Spello, and Foligno), so coming from Florence take one of the dozen
daily trains to Terontola/Cortona (1'/2 hr.) that meet up with a
connecting train to Assisi/S.Maria degli ANgeli (45 to 60 min.).
From the South, the main line between Rome and Ancona has a station at
Foligno (on the branch line to Spello, Assisi, Perugia and Lake
Trasimene), so coming from Rome, take one of the nine daily trains on the
line to Ancona, stop at Foligno (1 hr., 40 min. to 2 hr.), where you can
transfer to a Perugia-bound train (10 to 15 min.).
All the cited lines are part of the FS state-run railway system and
stop at a station on Santa MAria degli Angeli, well
away from the historic center (about 3km)
but a 30-minute ride on buses from the center of
town (C Line). Bus services leave from the forecourt outside
the station and drop you in Piazza Matteotti.
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